Table 1.

Composition and structure of presently studied aLUVs and scrambled LUVs

	Compositiona			aLUV		Scrambled		$\Delta A/A$ (%)d
	${\chi }^{\text{total}}$	${\chi }^{\text{in}}$	${\chi }^{\text{out}}$	$A_{\text{av}}$ (Å2$)$b	$D_B$ (Å)c	A (Å2)b	$D_B$ (Å)c
POPEin/ESMout	61:39	98:02	28:72	64.0	37.6	61.8	38.2	3.6
POPEin/MSMout	67:33	82:18	54:46	61.4	40.3	63.8	39.0	−3.8
POPEin/POPCout	77:23	90:10	65:35	62.0	39.0	64.9	37.4	−4.5
POPEin/(POPC/MSM)out	52:48	65:35	40:60	62.9	39.6	65.0	38.4	−3.2
(POPE/POPS)in/MSMout	59:41	97:03	25:75	62.8	40.0	64.3	39.1	−2.3
(POPE/POPS)in/POPCout	71:29	97:03	48:52	66.0	37.2	65.8	36.7	0.3
(POPE/POPS)in/(POPC/MSM)out	62:38	77:23	49:51	63.4	39.1	66.9	37.1	−5.2

^aχ, molar ratio between acceptor and donor lipids.

^bA, lateral area per lipid. Average between inner and outer leaflet for aLUVs. Estimated relative uncertainty, 2%.

^c$D_B$, Luzzati bilayer thickness. Estimated relative uncertainty: 2–3%.

^d$\Delta A/A=\left(A_{av}^{\text{aLUV}}-A^{\text{scrambled}}\right)/A^{\text{scrambled}}$. Estimated absolute uncertainty: 4%.