Figure 6.

Effect of fasting on SGLT1 and non-electrogenic glucose mediated absorption mechanism in the jejunum. In chambers containing a mucosal preparation from the jejunum (S1), the Ringer’s solution of mucosal (M) side (A, B) or serosal (S) side (C, D) was replaced with a Ringer’s solution containing 30 mM Methyl α-d-glucopyranoside (nonmetabolizable analog of glucose, MGP) and labeled with ¹⁴C-MGP at time 0. The unidirectional flux (J_{M to S}, J_{S to M}) of MGP was calculated by collecting a sample from the cold side every 20 min. I_sc was measured simultaneously under short circuit conditions (A′, B′). Where indicated by arrows, 100 µM phloridzin was added to the mucosal side (at 60 min). Data are the mean ± standard deviation (SD). n = 5. P values are shown with numbers, One-way ANOVA.