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. 2023 Jul 5;13:10838. doi: 10.1038/s41598-023-38024-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of fasting on mRNA expression of claudins. The effect of fasting on Cldn2 (A), Cldn15 (B) and Cldn7 (C) mRNA expression. Data are normalized to beta-actin. Data are presented as mean ± SD. Numbers in parentheses indicate the number of animals. P values are shown with numbers, Two-way ANOVA. Effect of fasting on tissue distribution of claudin-2 (A′), claudin-15 (B′) and claudin-7 (C′). To demonstrate the specificity of the signal, DIC images were obtained at the same time.