REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
TruStain FcX™ (anti-mouse CD16/32) Antibody Clone: 93 (concentration: 0.5 mg/mL)	BioLegend	Cat#101320 RRID: AB_1574975
Alexa Fluor 488 anti-mouse F4/80 Antibody Clone: BM8 (concentration: 0.5 mg/mL)	BioLegend	Cat#123120 RRID: AB_893479
Alexa Fluor 647 anti-mouse CD206 (MMR) Antibody Clone: C068C2 (concentration: 0.5 mg/mL)	BioLegend	Cat#141712 RRID: AB_10900420
APC Hamster Anti-Mouse CD11c Clone: Clone HL3 (concentration: 0.2 mg/mL)	BD Biosciences	Cat# 550261 RRID: AB_398460
Chemicals, peptides, and recombinant proteins
Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg/mL Solution in Water	Invitrogen	Cat# H3570
ProLong™ Gold Antifade Mountant	Invitrogen	Cat# 36930
16% Paraformaldehyde aqueous solution, EM grade, ampoule 10 mL	Electron Microscopy Sciences	Cat# 15710
Tissue-Tek® O.C.T. Compound	Sakura® Finetek	Cat# 4583
2-Methylbutane - isopentane	Sigma-Aldrich	Cat# M32631 CAS# 78-78-4
Acetone, histological grade, ≥99.5%	Sigma-Aldrich	Cat# 534064 CAS# 67-64-1
Normal serum (Rat)	Jackson ImmunoResearch Inc.	Cat# 012-000-120 RRID: AB_2337141
Ammonium chloride	Sigma-Aldrich	Cat# A4514 CAS# 12125-02-9
Ketamine (80 mg/kg)	VetOne	VINB-KETO-7021
Xylazine (10 mg/mL)	Bimeda Animal Health Inc.	1XYL006 sku: 061035
Experimental models: Organisms/strains
Rosa26floxed-tdTomato	The Jackson Laboratory	Jax Strain # 007909
Cx3cr1CreERT2-IRES-YFP	The Jackson Laboratory	Jax Strain #020940
B6.Cg-Tg(Vil1-cre)997Gum/J - VillinCre	The Jackson Laboratory	Jax Strain #004586
Software and algorithms
LAS Advanced Fluorescence Software	Leica Microsystems	https://www.leica-microsystems.com
Nikon’s NIS-Elements software package	Nikon	www.microscope.healthcare.nikon.com
Orca Flash 4.0 mounted digital camera	Hamamatsu Photonics K.K.,	https://www.hamamatsu.com
Other
Conventional fluorescent microscope	N/A	Leica DM6000B fluorescence microscope
Confocal microscope	Nikon	Nikon A1R SI confocal microscope with the DUG GaAsP detector
Benchtop HDPE Dewar Flasks	Thermo Scientific Nalgene	Cat# 4150
Cryostat	Leica	Cat# CM1950
Mini Nutator Rocker	Benchmark	Cat# H3D1020
Essendant Royal Wood Toothpicks	Fisher Scientific	Cat# NC1678836
Straight reusable feeding needles 18G	VWR	Cat# 20068-620
Disposable Syringes with Luer-Lok™ Tips	BD Medical	Cat# 309657
Superfrost™ Plus Microscope Slides	Fisher Scientific	Cat# 12-550-15
Micro Cover Glass 22 × 22 mm No. 1	VWR	Cat# 48366-067
Disposable Petri dishes	VWR	Cat# 25384-088
Slide moisture chambers for IHC staining, black	VWR	Cat# 76278-848
Peel-A-Way® Disposable Embedding Molds, Polysciences, Square Mold	VWR	Cat# 15160-215
Grade GB003 blotting paper (Whatman paper)	VWR	Cat# 28152-032
Hardened fine scissors, stainless steel, curved, 9 cm	Fine Science Tools	Cat# 1409109
Forceps, 4" (10 cm) long, 0.8 mm tip, serrated, full curve	Roboz	Cat# RS-5137
Stainless steel tongs with silicone tips; 12″	Cole-Parmer Essentials	Cat# EW-06443-08