Figure 5.

De Novo Genome Assembly Reveals Novel Pig Genes, Transcripts, and Infarct Biology and Shows That PDGF-AB Accelerates Scar Maturation and Reduces Inflammatory Cytokines in Pigs Post-MI

(A, B) Number of identified genes and transcripts for current pig genome annotation (SScrofa11.1) and our de novo library assembly. (C) DE genes for control pig 11 days post-MI cardiac tissue vs shams using our de novo library assembly. (D) Volcano plots showing DE genes in control pigs (vs shams). Red dots represent genes that met both P value and log₂(fold change) cutoffs. (E) GSEA for control DE genes in control pigs (vs shams). (F) Heatmap of PDGF-AB–treated pig RNAseq for DE ECM-related genes. (G) Picro-Sirius red histological staining of collagen fibers (red) in pig post-MI infarct zone (IZ, scale bar = 50 μm). (H) IHC of col1α1 scar (grey), CD3⁺ T cells (red, arrows), and FXIIIa⁺ macrophages (blue, arrowheads) in pigs post-MI. Scale bar = 2 mm (top) and 100 μm (bottom). (I to K) IHC image analyses of pig infarct zones (∗P < 0.05). (L) Heatmap of PDGF-AB–treated pig RNAseq for DE immune-related genes. Abbreviations as in Figures 1, 2, 3, and 4.