Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 5;11:35. doi: 10.1038/s41413-023-00269-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Degeneration of cell renewal in Prx1^Cre;Z24^fl/fl mice leads to bone loss. a Western blotting analysis of the expression of P16 in bone tissues of Prx1^Cre;Z24^fl/fl mice and their littermate controls. Tubulin was used as a reference protein. b Calcein double staining and mineral appositional rate (MAR) analysis of 16-week-old Prx1^Cre;Z24^fl/fl, Osx^Cre;Z24^fl/fl and their littermate controls. Scale bars, 20 μm. Data shown are from four replicates. c Masson staining of femur hard slides. Scale bars, 80 mm. Quantification of the number of osteoblasts per tissue area (N.Ob/T.Ar) (d) and osteoblast surface per bone surface (Ob. BS) (e). f Immunofluorescence images of cleaved Caspase-3 in the metaphysis and diaphysis. Scale bars, 25 μm. g Proportion of cleaved Caspase-3⁺ cells to Prx1^Cre; Rosa26^Ai9 cells in tissue sections. h Immunofluorescence images of SA-β Gal in the growth plate. SOC secondary ossification center, Po periosteum. Scale bars, 100 μm. i Proportion of SA-β Gal⁺ cells to Prx1^Cre; Rosa26^Ai9 cells in tissue sections. The statistical significance of differences was assessed using two-tailed Student’s paired t test (*P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.000 1)