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. 2023 Jul 5;11:35. doi: 10.1038/s41413-023-00269-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Acan^CreER;Z24^fl/fl mice exhibit trabecular bone loss. a Violin plots showing the expression levels and distribution of Acan. b Fluorescence images of OPN in femur sections from Acan^CreER; Rosa26^Ai9 mice that were injected with tamoxifen at p1-p3. Mice were analyzed after 5 weeks. Scale bar, 0.5 mm. c Fluorescence images of CD73 in the growth plate and Sca1 in the periosteum of 16-week-old Acan^CreER; Z24^fl/fl; Rosa26^Ai9 mice and Acan^CreER; Z24^fl/+; Rosa26^Ai9 mice. Scale bars, 50 μm. d FACS analysis of the distribution of Acan⁺CD73⁺ cells from growth plates of 8-week-old Acan^CreER; Z24^fl/+; Rosa26^Ai9 and Acan^CreER; Z24^fl/fl; Rosa26^Ai9 mice that were injected with tamoxifen at p1-p3. n = 3 per group. Data are presented as the mean ± SD. e MicroCT images showing femurs of 16-week-old Acan^CreER; Z24^fl/fl mice and their littermate controls. Scale bars, 1 mm. Quantitative analyses of trabecular bone volume/total volume (BV/TV) (f), trabecular thickness (Tb.Th) (g), trabecular spacing (Tb.Sp) (h), trabecular number (Tb.N) (i) and cortical thickness (Ct.Th) (j) in the femur metaphysis of 16-week-old Acan^CreER; Z24^fl/fl mice (n = 4) and their littermate controls (n = 6). The statistical significance of differences was assessed using two-tailed Student’s paired t test (*P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.000 1)