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. 2023 May 7;114(7):3014–3026. doi: 10.1111/cas.15821

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© 2023 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Physiological characteristics of the two clusters of osteosarcoma subtypes. (A) Heatmap showing the differences in enrichment in different gene sets between the two clusters. The deeper the red, the higher the enrichment. (B) Violin diagram showing the infiltration of 22 immune cells in two clusters. (C) Scatter plots shows immune score, stromal score, and ESTIMATE score of two clusters. (D) Boxplot showing the expression of human leukocyte antigen genes in two clusters. (E) Boxplot showing the expression of T‐cell stimulating factor genes in two clusters. (F) Heatmap showing 40 statistically significant drugs between cluster 1 and cluster 2. (G) Boxplot showing IC₅₀ values of two clusters against classic osteosarcoma chemotherapy drugs. *p < 0.05, **p < 0.01, ***p < 0.001.