Table 1.

Significantly altered genes along with both corresponding p-values and fold regulation.

Phenomena	5h ischemia vs. 1h ischemia			1h ischemia + AAT vs. 1h ischemia			5h ischemia + AAT vs. 5h ischemia			5h ischemia + AAT vs. 1h ischemia + AAT
	Symbol	p-Value	Regulation	Symbol	p-Value	Regulation	Symbol	p-Value	Regulation	Symbol	p-Value	Regulation
Apoptosis and cell death	Aifm1	0.014	-1.30	Aifm1	0.009	-1.29				Bax	0.008	-1.10
	Bad	0.003	-1.54
	Bcl2	0.010	-1.13
	Casp3	0.002	1.86
	Casp9	0.034	-1.28
	Cflar	0.044	1.81
	Cycs	0.009	-1.25
	Fadd	0.045	1.92
	Fos	0.014	2.29
Oxidative stress	Ccs	0.006	-1.31	Nox4	0.033	-1.65	Cyba	0.022	1.40	Cyba	0.022	1.36
	Gpx1	0.022	-1.18							Txnrd2	0.025	-1.25
	Gpx3	0.005	-1.37
	Gpx4	0.019	-1.15
	Gpx7	0.009	-1.80
	Gstk1	0.003	-1.25
	Hspa1a	0.004	3.70 B
	Ncf1	0.025	-1.28
	Nox4	0.005	-1.66
	Prdx1	0.003	-1.21
	Prdx2	0.028	-1.14
	Prdx3	0.043	-1.14
	Sod3	0.039	-1.34
	Txnrd1	0.002	2.33
Inflammatory response	Ccl2	0.009	3.92	Ccl20	0.044	1.68	Ccl11	0.013	3.00 B	Ccl20	0.006	-2.05
	Icam1	0.045	2.40	Vegfc	0.040	-1.47	Vcam1	0.041	-2.35	CxCr4	0.025	1.67
	Tlr4	0.002	-5.85 A
	Tollip	0.026	1.24
	Vegfc	0.022	-1.48

The significance cut-off criteria were set at p-value less than 0.05 with a fold regulation greater than 2.0 or less than 2.0. A fold regulation value above 2 denotes an increase in gene expression, while a value below -2 suggests a decrease. Refer to Online Table 1 for gene abbreviations and Online Table 2 for all 88 tested genes. Genes are categorized as “A” indicating that their average threshold cycle is relatively high (> 30) in either the control or test sample but reasonably low in the other sample (< 30); “B” indicating that their average threshold cycle is relatively high (> 30), meaning that their relative expression level is low in both control and test samples, and the p-value for the fold-change is either unavailable or relatively high (p > 0.05). AAT indicates alpha-1-antitrypsin.