Table 1. .

Characteristics of each high-sensitivity in situ hybridization method

Method		DIG-RNA ISH	RNAscope	HCR ISH	clampFISH	SABER FISH
Difficulty of experimental procedures		difficult	easy	moderate	moderate	moderate
Coloration Method		fluorescent chromogenic	fluorescent chromogenic	fluorescent	fluorescent	fluorescent
Multiplex staining		difficult under some conditions	easy	easy	easy	easy
Probe design and synthesis		done by user (can be outsourced)	provided by manufacturer only	done by user (can be outsourced)	done by user	done by user
Automated staining		applicable	applicable	—	—	—
Monetary cost	total	low	high	moderate	moderate	moderate
	per sample	low	high	decreases with increasing sample size	decreases with increasing sample size	decreases with increasing sample size
Time cost	examination of experimental conditions	necessary	mostly unnecessary	necessary	necessary	necessary
	staining time	2–3 days	1 day	1–3 days	1–3 days	2–3 days
Detection of microRNA		difficult	applicable	applicable	—	—

—: not reported.