Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 30;150(13):dev201484. doi: 10.1242/dev.201484

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 2. — Snr1 is required for the transition from neuroepithelial cells to neuroblasts. (A) Timeline of larval development showing timing of clonal induction and dissections after larval hatching (ALH). (B-C′) Snr1 mutant clones in the optic lobe have altered location and morphology. (B,B′) Control MARCM clone at surface of brain imaged in cross-section. (C,C′) Snr1^R3 MARCM clone located deeper inside the brain showing ectopic expression of Deadpan (Dpn; arrowheads). Cell junctions are marked by DE-cad, neuroblasts are marked by Dpn, MARCM clones are marked by GFP and GMCs are marked by Pros. (D-E″) Snr1 mutant clones deep in the medulla express markers for neuroepithelial cells and neuroblasts. (D-D″) Control optic lobe MARCM clone marked by GFP. Clone is outlined by a white dashed line. (E-E″) Snr1^R3 optic lobe MARCM clone marked by GFP. Clone is outlined by a white dashed line. Neuroepithelial cells marked by PatJ (red) and neuroblasts marked by Dpn (white). (F-F′) Snr1^R3 clone cells on the surface of the brain are smaller (arrowheads) and express neuroepithelial (NE) cell marker. Neuroepithelial cell junctions are marked by PatJ (magenta) and MARCM clones are marked by GFP (green). (G-H‴) MARCM clones imaged in cross-section at mid third instar stage. Brackets indicate transition zone. Arrows indicate ectopic Dpn⁺ cells. Scale bars: 20 µm.