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. 2023 Apr 21;10(19):2300880. doi: 10.1002/advs.202300880

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© 2023 The Authors. Advanced Science published by Wiley‐VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Islet organoids culture and GSIS experiment in vitro. A) Schematic illustration of pancreatic islet‐like organ culture and GSIS experiment. B) Immunofluorescent staining of Calcein‐AM (green) staining, PI (red) staining, and their merge images of live/dead islet β cells from each group. Scale bar: 200 µm. C) Cell viability of islet β cells from each group. D) Stimulation index of GSIS experiments of pancreatic islet‐like organs from each group. Data represent means ± S.D. from at least three independent replicates. (^## p < 0.01, ^### p < 0.001 vs Control group; **p < 0.01, ***p < 0.001 vs PA group).