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Pancreatic islets of Langerhans. Rat pancreatic islets were grown for 6 weeks on a layer of matrigel in the presence of RPMI-1640 medium containing 6 mM glucose and 5% FCS. The islets embedded in the extracellular matrix showed a smooth three-dimensional structure; by day 3, no contaminating fibroblasts could be seen on the surrounding extracellular matrix. The picture was taken under 100× magnification using a phase-contrast microscope.
