Fig. 6.

circ‐TNRC6B suppresses proliferation, migration, and invasion abilities of ESCC cells via miR‐452‐5p. (A, B) KYSE150 cells were transfected with negative control plasmid, circ‐TNRC6B siRNA alone, or co‐transfected with circ‐TNRC6B siRNA and miR‐452‐5p inhibitor. CCK‐8 (n = 4, P‐values were determined by two‐way ANOVA) and colony formation assay (n = 3, P‐values were determined by one‐way ANOVA) showed silencing circ‐TNRC6B alone significantly promoted the proliferation and clonogenic capacity of KYSE150 cells, and the above promoting effect of circ‐TNRC6B siRNA could be partially reversed by miR‐452‐5p inhibitor. (C–E) Wound‐healing and transwell assay indicated silencing circ‐TNRC6B alone remarkably increased the migration and invasion abilities of KYSE150 cells, while simultaneous knockdown of miR‐452‐5p could partially restore such promoting effects. Data are represented as mean ± SD (n = 3, P‐values were determined by one‐way ANOVA). Scale bar: 100 μm. **P < 0.01, ***P < 0.001.