Fig. 3.

LncRNA regulation through p53‐p21‐DREAM/RB signaling. Data on E2F4, p130/p107, and RB binding from ChIP‐seq datasets [45] at the genomic loci of the recurrently down‐regulated lncRNAs (A) THAP9‐AS1 and (B) OIP5‐AS1. Transcripts per million (TPM) values obtained from RNA‐seq data of Nutlin‐3a (orange) and DMSO control‐treated (gray) cells for (C) CDKN1A (p21) and MDM2 and (D) 17 lncRNAs recurrently down‐regulated by p53 and bound by DREAM/RB. (C, D) RNA‐seq data from parental HCT116 [9] and HCT116 p21^−/− cells [8]. Bar graphs display mean and standard deviation. TPM values from RNA‐seq datasets were compared using a two‐sided unpaired Student's t‐test. *, **, ***, and n.s. indicate P values < 0.05, < 0.01, < 0.001, and > 0.05, respectively.