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. 2023 Mar 16;69(1):45–56. doi: 10.1165/rcmb.2022-0292OC

Figure 1.

Figure 1.

Matrix contribution by lung fibroblast subpopulations in mouse fibrotic lungs. (A–J) Single-cell transcriptomics analysis of the adult mice (A–E) or aged mice (F–J) with bleomycin-induced lung fibrosis. (K–O) Single-cell transcriptomics analysis of mice with silica-induced lung fibrosis. (A, F, and K) Distribution of the mesenchymal cells from normal and fibrotic lungs of adult mice (A) and aged mice (F) in bleomycin- and silica-induced (K) lung fibrosis models. (B, G, and L) Identification of the fibroblast subtypes in lungs from adult (B) or aged (G) mice in bleomycin- and silica-induced (L) lung fibrosis models. (C, H, and M) Percentage of fibroblast subtypes in lungs from adult (C) or aged (H) mice in bleomycin- and silica-induced (M) lung fibrosis models. (D, I, and N) Violin plot comparisons of the collagen score (average expression of the matrix genes: Col1a1, Col1a2, Col3a1, and Fn1) in lung fibroblasts of adult (D) and aged (I) mice in bleomycin- and silica-induced (N) lung fibrosis models. (E, J, and O) Violin plot comparisons of the collagen score in fibroblast subtypes of adult (E) and aged (J) mice in bleomycin- and silica-induced (O) lung fibrosis models. Wilcoxon P < 0.05 is statistically significant by Wilcoxon rank sum test (D, E, I, J, N, and O). Ebf1+ = Ebf1+ fibroblast; Inter = intermediate fibroblast; Lipo = lipofibroblast; Meso = mesothelial cell; Myo = myofibroblast; Peri = pericyte; SM = smooth muscle cell; UMAP = uniform manifold approximation and projection.