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. 2023 Mar 16;69(1):45–56. doi: 10.1165/rcmb.2022-0292OC

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Copyright © 2023 by the American Thoracic Society

This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives License 4.0. For commercial usage and reprints, please e-mail Diane Gern.

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Figure 3. — Identification of cell surface marker genes of each fibroblast subpopulation in human lungs. (A) Cell surface marker genes were identified in the integrated single-cell RNA-sequencing data of mesenchymal cells from IPF and healthy human lungs: CD82 for lipofibroblasts, BCAM (CD239) for myofibroblasts, CD55 for EBF1⁺ fibroblasts, PDGFRB (CD140B) for pericytes, and VCAM1 (CD106) for mesothelial cells. (B and C) Costaining of CD82 (lipofibroblast marker) and HTII-280 (human alveolar type II cell marker) (B) and α-SMA (α-smooth muscle actin) (myofibroblast marker) (C) on healthy and IPF lung sections. (D) Bright-field and fluorescence images of the CD82^neg (nonlipofibroblasts) and CD82^hi (lipofibroblasts) fibroblasts after visualization of the intracellular lipid droplets by Nile red staining. Higher magnifications of the boxed regions are shown (B–D). Scale bars, 50 μm (B and D).