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. Author manuscript; available in PMC: 2023 Jul 6.
Published in final edited form as: Neurobiol Dis. 2023 Mar 29;181:106111. doi: 10.1016/j.nbd.2023.106111

Fig. 5.

Fig. 5.

Despite equivalent SN DA neuron depletion and striatal vector transduction, CAV Responders demonstrate decreased parenchymal Cacna1d when compared to Non-Responders. (A) CAV Responders (Res (n = 7)) show a significantly more negative LID fold change than Non-Responders (NonR (n = 7)) (Mann-Whitney U test, p = 0.006). The LID fold change shown was defined at the moderate levodopa dose, 6 mg/kg (pre-vector D20 vs. post-vector D55). (B) The number of estimated DA (TH+) neurons in the rat SN did not differ in either the intact contralateral or lesioned ipsilateral hemisphere between Res and NonR (unpaired, two-tailed t-tests, p > 0.05). All animals were unilaterally lesioned, with >95% loss of TH+ neurons on the ipsilateral side. (C) Percent striatal transduction did not differ between Res and NonR (unpaired, two-tailed t-test, p > 0.05). (D) Cacna1d fold change in GFP+ vector-transduced cells (normalized to contralateral HuC/D+ cells) did not differ between Responders and Non-Responders (unpaired, two-tailed t-test, p > 0.05). (E) Cacna1d fold change in HuC/D+ non-transduced cells did not differ between Responders and Non-Responders (unpaired, two-tailed t-test, p > 0.05). (F) CAV Responders compared to Non-Responders showed significantly greater downregulation of total striatal Cacna1d as measured by DAB+ transcript area (unpaired, two-tailed t-tests, p = 0.049). All bars represent group mean; error bars show ± SEM.