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. Author manuscript; available in PMC: 2023 Jul 6.
Published in final edited form as: Alcohol Clin Exp Res (Hoboken). 2023 Feb 17;47(3):470–485. doi: 10.1111/acer.15019

FIGURE 5.

FIGURE 5

Indomethacin rescues basal forebrain cholinergic neuron number after Adolescent intermittent EtOH (AIE) in both sexes. (A) AIE-reduced ChAT + IR basal forebrain cholinergic neurons by 33% in males (blue) and 11% in females (pink). Indomethacin rescued this effect in both sexes, restoring ChAT + IR cell number to control values in both sexes. Data are expressed as mean ± SEM. (B) Photomicrographs of ChAT labeling of cholinergic neurons in the medial septum of the basal forebrain. For example, photomicrographs were taken at 40× magnification. Fewer ChAT + IR neurons are evidenced in AIE–vehicle-treated rats, but indomethacin restored ChAT + IR cell number after AIE, suggesting that AIE-induced apparent cholinergic cell loss is reversible through anti-inflammatory interventions in adulthood. Scale bar is 50 μm.