Fig. 1.

Cyclization verification, subcellular localization, and expression pattern analysis of circDOCK7. A Schematic diagram showing the formation and designing of primers for cyclization verification of circDOCK7. B PCR amplification of the divergent and convergent primers of circDOCK7 using gDNA and cDNA as templates, respectively. C Confirmation of the backsplicing junction of circDOCK7 via Sanger’s sequencing. D qRT-PCR analysis of the relative expression of circDOCK7 and DOCK7 in chicken abdominal preadipocytes treated with RNase R. The relative gene expression levels are shown as fold changes compared with that in the RNase R − group. E qRT-PCR analysis of the relative expression of circDOCK7 and DOCK7 in chicken abdominal preadipocytes treated with actinomycin D. The relative gene expression levels are shown as fold changes compared with that in the 0 h group. F qRT-PCR analysis of circDOCK7 expression in the cytoplasm and nuclear fractions of chicken abdominal preadipocytes. G Subcellular localization of circDOCK7 in chicken abdominal preadipocytes using RNA fluorescence in situ hybridization assay. Cell nuclei were stained with 4′,6-diamidino-2-phenylindole DAPI (blue), and circDOCK7 was hybridized with the circDOCK7 probe (green). H Tissue expression pattern of circDOCK7 in chickens with high and low abdominal fat percentage. I and J Expression pattern of circDOCK7 during the proliferation and adipogenic differentiation of chicken abdominal preadipocytes. During the proliferation of chicken abdominal preadipocytes, the relative expression levels are shown as fold change versus that of the 24 h group, and the statistical significance analysis are performed versus the 24 h group as control. During the adipogenic differentiation of chicken abdominal preadipocytes, the expression levels are shown as fold change versus that of the 0 h group, and the statistical significance analysis are performed versus the 0 h group as control.  ^*P < 0.05, ^**P < 0.01. The same below