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. 2023 Jul 6;14:91. doi: 10.1186/s40104-023-00891-8

Fig. 11.

Fig. 11

circDOCK7 augments adipogenesis in a gga-miR-301b-3p-dependent manner. A Relative expression of the ACSL1 gene in proliferative chicken abdominal preadipocytes co-transfected with sicircDOCK7 and miR-301b-3p antagomir. The relative gene expression levels are shown as fold changes compared with that in the siNC + antagomir NC group. BD CCK8 and EdU staining assays in proliferative chicken abdominal preadipocytes co-transfected with sicircDOCK7 and miR-301b-3p antagomir. E Relative expression of the ACSL1 gene in differentiated chicken abdominal preadipocytes co-transfected with circDOCK7 overexpression vector and miR-301b-3p agomir. The relative gene expression levels are shown as fold changes compared with that in the pLC5-ciR + agomir NC group. F–H Intracellular lipid droplet accumulation and triglyceride and acetyl-CoA levels in differentiated chicken abdominal adipocytes co-transfected with circDOCK7 overexpression vector and miR-301b-3p agomir. *P < 0.05, **P < 0.01. a–cMeans with different lowercase letters indicate significant difference (P < 0.05)