Fig. 3.

Effects of circDOCK7 on the adipogenic differentiation of chicken abdominal preadipocytes. A Detection of circDOCK7 overexpression after 48 h of transfection with circDOCK7 overexpression vector in differentiated chicken abdominal adipocytes. The relative gene expression levels are shown as fold changes compared with that in the pLC5-ciR group. The same below. B and C Representative images of Oil Red O staining and lipid droplet content of circDOCK7-overexpressing differentiated chicken abdominal adipocytes. D Representative images of Nile red fluorescent staining of circDOCK7-overexpressing differentiated chicken abdominal adipocytes. E and F Intracellular triglyceride and acetyl-CoA levels of circDOCK7-overexpressing differentiated chicken abdominal adipocytes. G Relative mRNA expression levels of PPARγ and CEBPα in circDOCK7-overexpressing differentiated chicken abdominal adipocytes. H Detection of circDOCK7 knockdown after 48 h of transfection with sicircDOCK7 in differentiated chicken abdominal adipocytes. I and J Representative images of Oil Red O staining and lipid droplet content of circDOCK7-knockdown differentiated chicken abdominal adipocytes. K Representative images of Nile red fluorescent staining of circDOCK7-knockdown differentiated chicken abdominal adipocytes. L and M Intracellular triglyceride and acetyl-CoA levels of circDOCK7-knockdown differentiated chicken abdominal adipocytes. N Relative mRNA expression levels of PPARγ and CEBPα in circDOCK7-knockdown differentiated chicken abdominal adipocytes. ^*P < 0.05, ^**P < 0.01