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. 2023 Jul 6;14:91. doi: 10.1186/s40104-023-00891-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — circDOCK7 functions as a gga-miR-301b-3p sponge. A and B Relative expression of gga-miR-301b-3p in proliferative and differentiated chicken abdominal preadipocytes upon circDOCK7 overexpression and knockdown. C and D Relative expression of the ACSL1 gene in proliferative and differentiated chicken abdominal preadipocytes upon circDOCK7 overexpression and knockdown. E Construction of dual-luciferase reporter vectors for the validation of gga-miR-301b-3p binding to circDOCK7. WT: wild-type vector; Mut: mutant vector; hRluc: Renilla luciferase; hluc + : firefly luciferase. F Validation of the interaction between gga-miR-301b-3p and circDOCK7 via a dual-luciferase reporter assay in 293 T cells and DF1 cells. G Detection of the combination of circDOCK7 and gga-miR-301b-3p by an RNA pull-down assay. H qRT-PCR analysis of gga-miR-301b-3p expression pulled down by the circDOCK7 probe. ^*P < 0.05, ^**P < 0.01