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. 2023 Jul 5;16:132. doi: 10.1186/s13048-023-01209-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Effects of IGF-1 on lamb GCs. a The expression of IGF-1 in lamb ovaries detected by immunohistochemistry (scale bar = 100 μm). b The relative mRNA levels of cell proliferation related genes detected by qRT-RCR. c The relative mRNA levels of cell apoptosis related genes detected by qRT-RCR. The cultured GCs were treated with 100 ng/ml IGF-1 for 12 h and then were harvested for detection of the gene expression. *P<0.05; **P<0.01; ***P<0.001