Figure 4. Estrogen increases the recruitment of myeloid cells through the secretion of CXCL1 and G-CSF.
Circulating pro-angiogenic factors, G-CSF from (A) A549 and (B) HCC827 & CXCL1 levels were quantified from (C) A549 and (D) HCC827 mice xenografts in the presence of estrogen. The samples were quantified in duplicate by using the Bio-rad multiplex bead assay. The results were plotted as average of the duplicate and graphed as mean ± SEM. Quantification of CD11b+ staining of A549 (E) and HCC827 (F) xenografts with and without estrogen treatment. A minimum of eight high power field (400X) was counted for CD11b+ cells from the periphery to the center of the tumor from each sample. The results were plotted as average of eight high power field (400X) and graphed as mean ± SEM.