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. 2023 Mar 7;29(7):1981–1998. doi: 10.1111/cns.14154

FIGURE 2.

FIGURE 2

SNI decreased the 50% paw withdrawal threshold, decreased the level of total bile acids, and upregulated CYP7A1 in microglia in the lumbar spinal dorsal horn. (A) SNI decreased the 50% paw withdrawal threshold on the ipsilateral hind paw on days 4, 7, and 14. **p < 0.01 compared with the sham group (Mann–Whitney U test, n = 6/group). (B) The level of total bile acids in the lumbar spinal dorsal horn on day 7 and day 14 after SNI. **p < 0.01 compared with the sham group. (C) Western blotting shows the expression of CYP7A1 in the ipsilateral lumbar spinal dorsal horn 7 and 14 days after SNI or sham operation. The quantification of CYP7A1 normalized to β‐actin is shown under the representative bands (n = 5/group). **p < 0.01 compared with the sham group. (D, F, and H) In the ipsilateral lumbar spinal dorsal horn, CYP7A1 was located mainly in Iba1‐labeled microglia (D) and to a much lesser extent in GFAP‐labeled astrocytes (F) but not in NeuN‐marked neurons (H) 7 days after either sham or SNI operation. (F, G and I), The percentage of microglia, astrocytes and neurons that expressed CYP7A1 in sham and SNI mice (day 7). Bar = 200 μm. ****p < 0.0001 compared with the sham group.