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. 2023 Feb 27;29(7):1830–1847. doi: 10.1111/cns.14142

FIGURE 2.

FIGURE 2

LncRNA INPP5F was downexpressed in RVLM after 15 days of stress exposure in rats. (A) Chronic stress increased the levels of SBP, MAP, and HR in rats. (B) Cluster analysis of the top five most increased and decreased lncRNAs identified by RNA sequencing in the control and SIH rats. (C) Relative expression of the four indicated lncRNAs listed in (B) measured by qRT‐PCR. (D) Relative expression of lncRNA INPP5F in 11 tissues of SIH rats, as determined by qRT‐PCR. (E) Bioinformatics prediction of the subcellular location of lncRNA INPP5F by lncLocator and iLoc‐LncRNA software. (F) Subcellular localization of lncRNA INPP5F in B104 cells using FISH. NC means Cy3‐labeled negative control probe. (G) qRT‐PCR was used to measure the expression of lncRNA INPP5F in either the nucleus or cytoplasm. Data were presented as mean ± SEM. Statistical significance was determined by two‐tailed unpaired Student's t‐test (A, C) and one‐way ANOVA, followed by post hoc Bonferroni test (D). n = 6 rats per group (A, C, D). n = 6 of independent cell culture preparations (G). *p < 0.05, **p < 0.01, and ***p < 0.001 versus control group. ### p < 0.001 versus heart group. DAPI, 4′,6‐diamidino‐2‐phenylindole; FISH, fluorescence in situ hybridization; HR, heart rate; MAP, mean arterial pressure; NC, negative control; qRT‐PCR, quantitative reverse transcription polymerase chain reaction; RVLM, rostral ventrolateral medulla; SBP, systolic blood pressure; SEM, standard error of the mean; SIH, stress‐induced hypertension.