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. 2023 Mar 21;29(7):1923–1939. doi: 10.1111/cns.14150

FIGURE 5.

FIGURE 5

ALOX15 is located downstream of DHODH and P53, and its expression is regulated by both, through in vitro and in vivo experiments. (A–C) ALOX15 expression and quantitative statistical analysis after up‐regulation of DHODH or (and) P53 was detected by Western blot in pc12 cells (n = 3). The expression of ALOX15 in the cells of each group was evaluated by immunofluorescence staining (n = 3, scale bar = 50 μm, the relative content of ALOX15 was calculated by Image J software). (D–F) In rats with spinal cord injury, western blot was used to detect and quantify the expression of ALOX15 in each group. Inhibition of DHODH up‐regulated ALOX15 expression (n = 10 for each group). The immunofluorescence staining of spinal cord injury rats was used to detect the immunofluorescence intensity of each group (n = 10 for each group, scale bar = 50 μm, relative content of ALOX15 was calculated by Image J software). (All the data are expressed as means ± SD, one‐way ANOVA followed by Tukey's post hoc test was applied. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Control; ^ p < 0.05, ^^ p < 0.01, ^^^ p < 0.001 vs. OE‐P53; *p < 0.05, **p < 0.01, ***p < 0.001 vs. SCI; & p < 0.05, && p < 0.01, &&& p < 0.001 vs. SCI + ferrostatin‐1.)