Table 1. MicroED statistics of proteinase K crystallized by suspended drop (PDB entry 8sdk and EMD-40351).
Data collection | |
Accelerating voltage (kV) | 300 |
Wavelength (Å) | 0.019687 |
Electron source | Field emission gun |
Total accumulated exposure (e− Å−2) | 0.64 |
No. of crystals | 1 |
Microscope | Thermo Fisher Titan Krios |
Camera | Falcon 4 electron counting |
Rotation rate (° s−1) | 0.2 |
Data analysis | |
Resolution range (Å) | 30.42–2.10 |
Space group | P43212 |
a, b, c (Å) | 68.26, 68.26, 101.95 |
α, β, γ (°) | 90, 90, 90 |
Total/unique reflections | 56301/12774 |
Multiplicity | 4.41 |
Completeness (%) | 87.0 |
Mean I/Iσ(I) | 2.82 |
CC 1/2 | 92.5 |
R work | 0.2442 |
R free | 0.2917 |