BLA astrocytes respond reliably to the initiation and termination of freezing behavior during contextual recall. A, Representative calcium time series (dF/F percentage) for shock and no-shock conditions during the 360 s recall session in the absence of foot shock. B–E, The z-scored dF/F (percentage) across recall for (B) shock and (D) no-shock conditions; each row represents a single subject across time within the session. Perievent analysis for the initiation (C) and termination (E) of freezing behavior, with each event occurring at the dashed line (time = 0). F, G, Behavioral analysis; average percentage freezing (F) and freezing across time within the recall session (G). H–K, Calcium event metrics; peak height (H), area under the curve (I), full-width half-maximum (J), and frequency (K). L, True and predicted traces produced from a generalized linear model. Error bars indicate SEM. For t tests and ANOVAs, p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; ns, Not significant. For perievent metrics, *95% CI, **99% CI; ns. For t tests and ANOVAs, shock n = 11, no shock n = 7. For freezing perievents, shock n = 11, no shock n = 6 because of a mouse not freezing during the recall session.