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. Author manuscript; available in PMC: 2023 Jul 6.
Published in final edited form as: Cancer Lett. 2020 Oct 9;496:127–133. doi: 10.1016/j.canlet.2020.10.007

Figure 2.

Figure 2.

PA promotes the phosphorylation of PTEN at T366. (A) U2OS cells were treated with 400 mM PA in the presence of MG132, and the level of PTEN phosphorylation was examined. (B) PTEN T366 phosphorylation of liver samples from five HFD fed mice and five controls were determined by IB analysis using corresponding antibodies. (C) U2OS cells were transfected with HA-PTEN, HA-T366A or HA-T366D expressing vectors, treated with PA or control, and the PTEN protein level was determined by IB analysis. (D) U2OS cells were transfected with His-ubiquitin together with wild-type or mutant PTEN. Ubiquitinated PTEN was pull downed by Ni2+-NTA beads and detected by HA antibody (E) The interaction between WWP2 and PTEN mutants was examined in U2OS cells by IP with anti HA antibody, followed by IB with WWP2 antibody after transfected with wild-type or mutant HA-PTEN expressing vectors.