Figure 3.

PA induces PTEN T366 phosphorylation via the mTOR/S6K pathway. (A) U2OS cells were treated with PA in the presence or absence of the mTOR inhibitor RAD001 as indicated. Activation of S6K was detected using S6K pT389 antibody (pS6K). (B) S6K phosphorylation of liver samples from five HFD fed mice and five controls were determined by IB analysis. (C) U2OS cells were treated with PA in the presence of MG132, RAD001, the S6K inhibitor NaSal or the GSK3β inhibitor TWS119. PTEN protein and T366 phosphorylation levels were detected by IB with the corresponding antibodies as indicated. (D) U2OS cells were treated with PA, with or without NaSal or RAD001. The interaction between endogenous WWP2 and PTEN was examined as indicated.