Table 1: Confocal and super-resolution microscopy acquisition and processing parameters for the representative images.
All of the main imaging parameters are compiled for the representative images provided.
| Figure Number | 1C | 2A | 3A | 4A | 4B | 5A | 5B | 6A | 6B | 6A | 6C | ||
| Acquisition Mode | Confocal | Confocal | Airyscan | Confocal | Airyscan | Confocal | Airyscan | Airyscan | Airyscan | Confocal | Airyscan | ||
| Objective | Plan-Apochromat 20x/0.8 M27 | Plan-Apochromat 63x/1.4 Oil DIC M27 | Plan-Apochromat 63x/1.4 Oil DIC M27 | Plan-Apochromat 63x/1.4 Oil DIC M27 | Plan-Apochromat 63x/1.4 Oil DIC M27 | Plan-Apochromat 63x/1.4 Oil DIC M27 | Plan-Apochromat 63x/1.4 Oil DIC M27 | Plan-Apochromat 63x/1.4 Oil DIC M27 | Plan-Apochromat 63x/1.4 Oil DIC M27 | Plan-Apochromat 63x/1.4 Oil DIC M27 | Plan-Apochromat 63x/1.4 Oil DIC M27 | ||
| Scan Mode | Frame | Frame | Frame | Frame | Frame | Frame | Frame | Frame | Frame | Frame | Frame | ||
| Image Size (Pixels) | 5172x5172 | 359x359 | 694 x 694 | 359x359 | 694 x 694 | 359 x 359 | 694 x 694 | 1040 x 1040 | 1040 x 1040 | 359 x 359 | 694 x 697 | ||
| Image Size (Scaled) | 638.90 μm x 638.90 μm | 25.35 μm x 25.35 μm | 24.51 μm x 25.51 μm | 25.35 μm x 25.35 μm | 24.51 μm x 25.51 μm | 25.35 μm x 25.35 μm | 24.51 μm x 25.51 μm | 36.71μm x 36.71 μm | 36.71μm x 36.71 μm | 25.35 μm x 25.35 μm | 24.51 μm x 24.51 μm | ||
| 640 | 1 au/ 31 μm | NA | NA | NA | NA | NA | NA | 5 au/ 281 μm | 5 au/ 281 μm | 1 au/ 56 μm | 5 au/ 281 μm | ||
| 561 | 1 au/ 50 μm | 1 au/ 50 μm | 5 au/ 247 μm | 1 au/ 50 μm | 5 au/ 247 μm | 1 au/ 50 μm | 5 au/ 247 μm | 5 au/ 247 μm | 5 au/ 247 μm | 1 au/ 50 μm | 5 au/ 247 μm | ||
| 488 | 1 au/ 24 μm | 1 au/ 44 μm | 5 au/ 219 μm | 1 au/ 44 μm | 5 au/ 219 μm | 1 au/ 44 μm | 5 au/ 219 μm | 5 au/ 219 μm | 5 au/ 219 μm | 1 au/ 44 μm | 5 au/ 219 μm | ||
| 405 | 1 au/ 21 μm | 1 au/ 38 μm | 5.46 au/ 205 μm | 1 au/ 38 μm | 5.46 au/ 205 μm | 1 au/ 38 μm | 5.46 au/ 205 μm | 5.46 au/ 205 μm | 5.46 au/ 205 μm | 1 au/ 38 μm | 5.46 au/ 205 μm | ||
| Scan Zoom | 0.5 | 4 | 4 | 4 | 4 | 4 | 4 | 2.7 | 2.7 | 4 | 4 | ||
| Pixel Time | 0.82 μs | 1.48 μs | 2.94 μs | 1.48 μs | 2.94 μs | 2.95μs | 2.94 μs | 0.99 μs | 0.73 μs | 2.17 μs | 1.08 μs | ||
| Frame Time | 3 min 24 sec | 1.78 s | 7.12 s | 1.78 s | 7.12 s | 3.57s | 7.12 s | 5.26s | 30.95 s | 2.62 s | 2.62 s | ||
| LSM Scan Speed | 5 | 9 | 6 | 9 | 6 | 7 | 6 | 7 | 8 | 8 | 8 | ||
| Scan Direction | Bidirectional | Bidirectional | Bidirectional | Bidirectional | Bidirectional | Bidirectional | Bidirectional | Bidirectional | Bidirectional | Bidirectional | Bidirectional | ||
| Averaging (Mean Intensity) | 2 | 2 | 1 | 2 | 1 | 2 | 1 | 1 | 1 | 2 | 1 | ||
| Bits per Pixel | 8 | 8 | 16 | 8 | 16 | 8 | 16 | 16 | 16 | 8 | 16 | ||
| Intensity (%) | 0.8 | NA | NA | NA | NA | NA | NA | 0.8 | 1.6 | 0.8 | 0.8 | ||
| Master Gain (V) | 700 | NA | NA | NA | NA | NA | NA | 840 | 803 | 700 | 810 | ||
| Intensity (%) | 0.8 | 0.7 | 0.8 | 0.7 | 0.8 | 0.8 | 0.8 | 0.9 | 1.4 | 0.8 | 0.9 | ||
| Master Gain (V) | 800 | 700 | 800 | 700 | 800 | 720 | 800 | 880 | 800 | 730 | 820 | ||
| Intensity (%) | 0.8 | 0.8 | 1 | 0.8 | 1 | 0.9 | 1 | 0.8 | 0.6 | 0.8 | 0.8 | ||
| Master Gain (V) | 810 | 800 | 900 | 800 | 900 | 820 | 900 | 830 | 800 | 840 | 900 | ||
| Intensity (%) | 0.8 | 0.7 | 0.9 | 0.7 | 0.9 | 0.8 | 0.9 | 0.9 | 0.6 | 0.5 | 0.9 | ||
| Master Gain (V) | 810 | 780 | 875 | 780 | 875 | 800 | 875 | 850 | 817 | 820 | 850 | ||
| Range | NA | 2.28 μm | 1.3 μm | 1.14 μm | 1.3 μm | 2.47 μm | 1.3 μm | 1.43μm | NA | 3.23 μm | 2.73 μm | ||
| Interval | NA | 0.19 μm | 0.13 μm | 0.19 | 0.13 μm | 0.190 μm | 0.13 μm | 0.13μm | NA | 0.19 μm | 0.13 μm | ||
| Slices | NA | 13 | 11 | (2–7) | (2–11) | 14 | 11 | 12 | 1 | 18 | 22 | ||
| Airyscan Processing (SR) | NA | NA | 7.3 | NA | 7.3 | NA | 6.4 | 7.1 | 7.1 | NA | 7.4 | ||