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. 2023 Jun 22;10:1164285. doi: 10.3389/fcvm.2023.1164285

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© 2023 Richert, Nienhaus, Ekroll Jahren, Gazdhar, Grab, Hörer, Carrel, Obrist and Heinisch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overview of the investigated culturing conditions. (A) examines the use of an incubator: (B) compares different medium exchange and refill schemes. (C) depicts the influence of growth time. (D) depicts the influence of prepreparation. Thickness is in mm.