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. 2022 Nov 11;119(8):1740–1750. doi: 10.1093/cvr/cvac160

Figure 2.

Figure 2

High salt intake amplifies the stress response. (A) protocol for measuring the stress response in male C57BL/6 mice (n = 20) fed 0.3% Na diet (control; open circles) and again after 2 weeks on a 3% Na diet (high salt; closed circles). (B) Plasma corticosterone in samples taken at 8am (baseline) and again after 15 min of tube restraint (Stress). Analysis was two-way ANOVA with repeated measures for the main effects of ‘stress-response’ (P < 0.0001) and ‘diet’ (P = 0.0006) and the interaction (P = 0.0014); P-values for planned comparisons (Holm–Sidak) are given. (C) The paired peak stress response in each mouse (n = 20), analysed by paired t-test. (D) The corticosterone recovery from stress was measured at either 30 (n = 6/6), 60 (n = 8/7) or 90 minutes (n = 5/6) following release from the restraint tube. Analysis was two-way ANOVA with repeated measures for the main effects of ‘time’ and ‘diet’ and the interaction; P-values for planned comparisons (Holm–Sidak) are given. Individual measurements are shown with group mean ± SD.