Figure 1. Thermal, monomeric and colloidal stability of formulated MS-Hu6 at increasing concentrations.

(A) Protein thermal shift assay confirmed thermostability of formulated MS-Hu6 between 0.2 mg/mL and 100 mg/mL. Compared with MS-Hu6 in PBS, the difference in melting temperature (ΔT_m) of the constant (Fc) and antigen-binding fragments (Fab) of formulated MS-Hu6 was <-1°C at all concentrations. (B) Size exclusion chromatography confirmed the monomeric nature of formulated MS-Hu6 between 1 and 100 mg/mL. Monomer loss was <1% in all concentrations. (C) Particle size distribution using dynamic light scattering yielded hydrodynamic radius (rh) and polydispersity index (PDI) of formulated MS-Hu6. Excellent colloidal stability (rh <10 nm, PDI <1) was found at concentrations from 1 to 100 mg/mL.