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. Author manuscript; available in PMC: 2024 Mar 15.
Published in final edited form as: J Am Chem Soc. 2023 Feb 16;145(10):5750–5758. doi: 10.1021/jacs.2c12367

Figure 1.

Figure 1.

Single-molecule fluorogenic reaction on AuNP@DNA. (a) Schematic of AuNP@DNA. A DNA hairpin binds to a 5 nm Au nanosphere through the two poly(adenosine) strands in the internal loop of its stem (see Supporting Information 3.1 for details). (b) N-deacetylation of Amplex Red (AR) generates a fluorescent product Resorufin (RF, emission ~583 nm), in a 50 mM pH 7.4 sodium phosphate buffer. (c) Scheme of the single-molecule reactivity measurement. Nanozymes are immobilized on the surface with reactants constantly flowing over. A 532 nm laser is configured in total internal reflection geometry to excite RFs generated on the nanozymes. An ultra-sensitive sCMOS camera captures the emission signals.