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Flowchart of different methods of formation of hybrid exosomes by fusion of lipid thin film with exosomes. Fusion with lipids can occur either by direct addition of exosomes for the hydration of the lipid thin film [190] or via the addition of exosomes to the preformed liposomes followed by freeze-thaw cycles [191], simple mixing and incubation [25] or sonication followed by extrusion [192].
