Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 6;14(7):404. doi: 10.1038/s41419-023-05919-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A, B CCK-8 and Transwell assays were used to determine the effect of MK-2206 (10 μM, DMSO as a control) on PLC/PRF/5-DAGLA cells. **P < 0.01, ***P < 0.001. C Western blotting analysis revealed that MK-2206 treatment (48 h) increased the p-LATS1 and p-YAP levels in PLC/PRF/5-DAGLA cells to some extent. D IF staining and Western blotting analysis (E) showed that MK-2206 slightly blocked YAP nuclear translocation in PLC/PRF/5-DAGLA cells. Scale bars, 10 μm. F Western blotting analysis was used to determine the effect of SC79 (10 μM, 1 h; DMSO as the control) on the levels of the indicated proteins in Hep3B-shDAGLA cells. G WB demonstrated that SC79 facilitated YAP nuclear transport, which was partially inhibited by DAGLA downregulation. H qRT–PCR confirmed the efficiency of cotransfection of YAP and DAGLA modulation constructs. I–L CCK-8 and Transwell assays were used to explore the effects of YAP OE or KD on Hep3B-shDAGLA or PLC/PRF/5-DAGLA cells, respectively. **P < 0.01. M WB indicated the regulation of the Hippo signalling pathway in cell lines cotransfected with YAP and DAGLA modulation constructs.