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. 2023 Jul 6;14(7):404. doi: 10.1038/s41419-023-05919-5

Fig. 6. PHLDA2 promotes HCC progression by facilitating cell proliferation and inducing the EMT process.

Fig. 6

A GSEA results of gene sets related to EMT, cell migration, liver cancer proliferation and cell cycle related in Hep3B-shDAGLA cells compared with the control group. B Heatmap of the expression of EMT- and cell migration-related genes in Hep3B-shDAGLA cells compared with the control group. C Heatmap of the expression of liver cancer proliferation- and cell cycle-related genes in Hep3B-shDAGLA cells compared with the control group. D The CDKN1C (p57) mRNA level was determined by qRT–PCR. E IF staining revealed the change in the E-cadherin levels in DAGLA-OE/KD cells. Scale bars, 10 μm. F Western blotting revealed the influence of DAGLA on the protein levels of key EMT mediators and p57. G Western blotting analysis clarified the effect of PHLDA2 on the expression of key EMT mediators and p57. H The levels of p57 and EMT-related proteins were verified by WB in PLC/PRF/5-DAGLA+PHLDA2-KD cells and Hep3B-shDAGLA+PHLDA2-OE cells. I Western blotting analysis showed the influence of exogenous 2-AG on EMT mediator expression in Hep3B-shDAGLA cells. J IHC staining demonstrated variations in the levels of p57, E-cadherin and vimentin in mouse xenograft tumours. Scale bars, 50 μm.