Table 2.
Cytochemical staining characteristics of leukocytes and platelets in Florida manatees (Trichechus manatus latirostris).
| n = 8 | ALP (n = 8) | ANBE (n = 8) | CAE (n = 8) | Luna (n = 3) | MPx (n = 8) | PAS (n = 3) | SBB (n = 8) | TB (n = 3) |
|---|---|---|---|---|---|---|---|---|
| Heterophils | G: +++ | – | G: + to +++ C: - to + (diffuse) | – | G: + to ++ | C: +++ (diffuse) | G: + to +++ | – |
| Eosinophils | G: ++ to +++ | – | – | G: +++ | G: +++ | C: ++ (diffuse) | +/−; G: + to ++ | – |
| Lymphocytes | – | C: ++ to +++ (multifocal; most); − (few) | +/−; G: + to +++ (multifocal) | – | – | – | – | – |
| Monocytes | +/−; C: + (few punctate) | +/−; C: + to ++ (focal to diffuse) | +/−; C: + to ++ (focal to diffuse) | – | +/−; C: + to +++ (punctate) | +/−; C: + (diffuse) | +/−; C: + to +++ (punctate) | – |
| Bilobed monocytes | +/−; C: + (few punctate) | +/−; C: + (diffuse) | +/−; C: + (diffuse) | – | +/−; C: + (punctate) | +/−; C: + (diffuse) | +/−; C: + to ++ (punctate) | – |
| Platelets | – | C: +++ (punctate) | – | – | – | C: + to +++ (focal to multifocal) | – | – |
Degree of staining was subjectively graded as positive (+), negative (−), equivocal (negative or weakly positive, +/−), weak (+), moderate (++) or strong (+++), in granules or cytoplasm, with the pattern of cytoplasmic staining further described as as punctate, focal, multifocal, or diffuse.
ALP, alkaline phosphatase; ANBE, alpha-naphthyl butyrate esterase; CAE, chloroacetate esterase; C, cytoplasm; G, granules; MPx, myeloperoxidase; PAS, periodic acid schiff; SBB, sudan black B; TB, toluidine blue. Erythrocytes were negative for each stain evaluated.