Figure 4.

Identification of putative transcription factors responsible for differential gene expression during Ot infection. Brain RNA-seq experiment was performed as in Figure 1 . (A) GSEA analysis to identify top 5 enriched transcription factors in mouse brain. (B) Enrichment plots for the transcription factor STAT2. (C) qRT-PCR assay confirms the upregulation of interferon-stimulated genes at day 10 as compared to mocks. A two-tailed student t test was used for comparison between two groups. *, p < 0.05; **, p < 0.01. The qRT-PCR experiments were performed twice independently with 4-5 samples per group, and the data are shown from a single representative experiment.