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. 2023 Jan 19;13(6):2715–2735. doi: 10.1016/j.apsb.2023.01.014

Figure 3.

Figure 3

D10 and D15 showed potent degradation effects on c-MET and inhibitory effects on p-c-MET. (A) The effects of tepotinib, D10 and D15 on c-MET and its downstream signaling pathways in EBC-1 cells. Cells were treated with D10 and D15 for 48 h at the indicated concentration. (B) The effects of D10 and D15 on c-MET and c-MET phosphorylation in EBC-1 cells. Cells were treated with D10 and D15 (10, 100 nmol/L) at indicated time. (C) EBC-1 cells were pretreated with D10 and D15 at 100 nmol/L) for 24 h, then washed with PBS three times, and harvested at the indicated time for Western blot analysis. (D) EBC-1 cells were treated with D15 at 10 nmol/L) for 48 h and then collected for liquid chromatography−mass spectrometry (LC−MS) analyses. The panel showed the relative abundance of c-MET (LFQ intensity value) between the degrader-treated and control groups.