Figure 7.

Corynoxine B (Cory B) promoted α-synuclein (α-syn) clearance in 2-, 10- and 15-month-old transgenic mice. (A–H, K, L) Prefrontal cortexes and midbrains of 2- and 10-month-old mice, and the midbrain of 15-month-old mice were sequentially extracted with Triton-X100-soluble and -insoluble buffers, as described in Materials and Methods. The expression levels of α-syn in each fraction were analyzed by Western blot. (J, L) Prefrontal cortexes of 15-month-old mice were extracted with RIPA lysis buffer (Medium) for Western blot analysis. Expression level of α-syn was assessed. (I) Representative images of α-syn pathology in the midbrain of 15-month-old mice in different groups immunolabeled with anti-α-synuclein phospho-(Ser129) antibody. Above quantifications are shown as mean ± SEM (^###P < 0.001 vs. normal group; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. model group, n = 5 mice).