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. 2023 Mar 25;13(6):2680–2700. doi: 10.1016/j.apsb.2023.03.019

Figure 6.

Figure 6

SIRT6 overexpression differentially regulates ATP production in cardiomyocytes and cancer cells during Dox treatment by enhancing mitochondrial respiration and inhibiting glycolysis. (A, B) Primary cardiomyocytes isolated from SIRT6 TG and WT littermates, LLC cells with lentivirus-mediated SIRT6 overexpression (Lenti-SIRT6) and control LLC cells (Lenti-Ctrl) were treated with Dox (0.5 μmol/L) for 12 h, and the oxygen consumption rate (OCR) was measured by a Seahorse XF96 extracellular-flux analyzer, (C, D) the lactate concentration was measured, (E–H) and the real-time ATP production rate assay was performed by a Seahorse XF96 extracellular-flux analyzer (n = 3 different experiments). The data are expressed as the mean ± standard deviation. One-way ANOVA followed by Bonferroni multiple comparisons were performed to determine significant differences. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 compared to the control group. #P < 0.05, ##P < 0.01, and ###P < 0.001 compared to the Dox-treated group.