FIGURE 5.

GCN5L1 directly binds to CypD and promotes the acetylation of CypD (A) Top 8 proteins were screened by the protein mass spectrometry and their potential interactions. (B) Co‐IP was used to show the interaction of GCN5L1 and CypD in WT and GCN5L1 HKO mice fed with HFHC or MCD. (C) IP was used to show the acetylation of CypD in WT and GCN5L1 HKO mice fed with HFHC or MCD. (D) Co‐IP was used to show the interaction of GCN5L1 and CypD in L02 cells transfected with control or MtG. (E) IP was used to show the acetylation of CypD in L02 cells transfected with control and MtG. (F) IP was used to show the acetylation of CypD in L02‐MtG cells transfected with WT or a mutation of CypD. (G) Cell viability was assayed in different indicated cells. (H–J) Malondialdehyde (MDA), lipid ROS and GSH were assayed in L02‐MtG cells with PA and transfected with WT or mutation of CypD. (K) HMGB1 was measured by ELISA in the serum of indicated groups.