REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Ubiquityl-PCNA (Lys164)	Cell Signaling Technology	RRID:AB_2798219
PCNA	Millipore	RRID:AB_11203836
α-tubulin	Sigma-Aldrich	RRID:AB_477583
Rad18	Abcam	RRID:AB_1603946
CHK1	Santa Cruz Biotechnology	RRID:AB_627257
CHK1pS345	Cell Signaling Technology	RRID:AB_331212
γH2AX	Cell Signaling Technology	RRID:AB_2118009
H2AX	Cell Signaling Technology	RRID:AB_10694556
Polη	Abcam	RRID:AB_2756352
Polκ	Atlas Antibodies	RRID:AB_2668606
Polι	Proteintech	RRID:AB_2167009
RPA	Abcam	RRID:AB_302873
CPDs	Cosmo Bio	RRID:AB_1962813
USP1	Proteintech	RRID:AB_2214314
Histone H2B	Abcam	RRID:AB_302612
Goat Anti-Rabbit Alexa 488	Thermo Fisher Scientific	RRID:AB_2576217
Goat Anti-Rabbit Alexa 568	Thermo Fisher Scientific	RRID:AB_10563566
Goat anti-Mouse Alexa 660	Thermo Fisher Scientific	RRID:AB_2535722
Goat anti-Mouse Alexa 660	Thermo Fisher Scientific	RRID:AB_2535722
Goat anti-Rabbit HRP	Cell Signaling Technology	RRID:AB_2099233
Goat anti-Mouse HRP	Cell Signaling Technology	RRID:AB_330924
Chemicals, peptides and recombinant proteins
Camptothecin	Sigma-Aldrich	C9911
Cisplatin	Sigma-Aldrich	P4394
DAPI	Sigma-Aldrich	D9542
Formaldehyde	Thermo Fisher	28908
Hydrogen Peroxyde	Sigma-Aldrich	216763
Sodium fluoride	Sigma-Aldrich	201154
β-Glycerophosphate	Sigma-Aldrich	G9422
Halt Proteases and Phosphatases inhibitors	Thermo Fisher Scientific	78440
Perm/Wash buffer	BD biosciences	554723
Prolong Diamond AntiFade	Thermo Fisher	P36961
RNAse A	EMD Millipore	70856
Benzonase	Sigma-Aldrich	E1014-5KU
BSA	Sigma-Aldrich	A2153
Critical commercial assays
BCA Protein Assay Kit	Thermo Fisher Scientific	23225
Click-iT EdU 488	Thermo Fisher Scientific	C10337
ECL Crescendo	Millipore	WBLUR0500
Experimental models: Cell lines
HCT166	ATCC	CCL-247
NCI-H1299	ATCC	CRL-5803
HEK293T	ATCC	CRL-3216
T98G	ATCC	CRL-1690
XP30RO	Masutani et al.22	N/A
XP30RO:Polη	Kannouche et al.13	N/A
XP30RO:GFP-Polη	Kannouche et al. 13	N/A
MEFs wild-type	Langerak et al.45	N/A
MEFs pcnaK164R	Langerak al.45	N/A
Oligonucleotides
Control siRNA (Luciferase)	5′-CACGUACGCGG AAUACUUCGATT-3′	N/A
Polη siRNA	Durando et al.46	N/A
Polι siRNA	Somyajit et al.23	N/A
Polκ siRNA	Bétous et al.47	N/A
Rad18 siRNA	Kermi et al.48	N/A
Recombinant DNA
pEGFP:Polη	Kannouche et al.13	N/A
pCDNA3-HA-FLAGpcnaK164R	This paper	N/A
Software and algorithms
Cell Profiler 4.2.1	Stirling et al.49	N/A
Kaluza	Beckman Coulter	N/A
Zen Blue	Zeiss	N/A
Prism	GraphPad	N/A
Other
4–15% Criterion™ TGX™ Precast Midi Protein Gel,	BioRad	5671085
Tris/Glycin Buffer	BioRad	1610771EDU
Trans-Blot Turbo Transfer System	BioRad	1704150
ChemiDoc	Biorad	N/A
Poly-D-Lysine	Gibco	A38904-01