Table 3.
Antibodies used in IMC
| Marker/target | Cell type | Functional state/specifics | Antibody clone(s) |
|---|---|---|---|
| ANXA1 | GBM cancer cells | Hypoxia driven mesenchymal | EPR19342/abcam |
| ANXA2 | GBM cancer cells | Hypoxia driven mesenchymal | MAB3928/RnD |
| BCAN | GBM cancer cells | Neural progenitor-like | S294A-6/Thermo |
| CD3 | Immune cells | T-cells | Fluidigm/3170019D |
| CD31 | Normal brain cells | Vasculature | Fluidigm/EPR3094 |
| CD45 | Immune cells | Pan-immune marker | Fluidigm/3152016D |
| CD8 | Immune cells | T-cells | SK1/Biolegend |
| CHI3L1 | GBM cancer cells | Mesenchymal | EPR19078-157/abcam |
| DNA | All cells | Cell nucleus | Fluidigm |
| DLL3 | GBM cancer cells | Neural progenitor-like | EPR22592-18/abcam |
| EZH2 | All cells | Chromatin remodeler | EPR9307(2)/abcam |
| GFAP | Normal brain cells | Astrocyte | ab218309/abcam |
| HIF1A | All cells | Hypoxia | 16H4L13/Thermo |
| HOPX | GBM cancer cells | Astrocyte-like | ab230544 |
| IBA1 | Immune cells | Pan-macrophage | EPR16588/abcam |
| JARID2 | All cells | Chromatin remodeler | Developed in house |
| JARID2 | All cells | Chromatin remodeler | EPR6357/abcam |
| Ki67 | All cells | Proliferating cells | B56/Fluidigm |
| MOG | Normal brain cells | Oligodendrocytes | MA5-24644/Thermo |
| NCAM (CD56) | Normal brain cells | Immature Neuron | HCD56/Biolegend |
| NeuN | Normal brain cells | Mature Neuron | 1B7/Biolegend |
| NKp46 | Immune cells | NK-cells | MAB1850/RnD systems |
| OLIG1 | GBM cancer cells | Oligodendrocyte progenitor-like | MAB2417/R&D |
| P2Y12R | Immune cells | Microglia | EPR23511-72/abcam |
| SCD5 | GBM cancer cells | Oligodendrocyte progenitor-like | PA5-59963/Thermo |
| SLC1A3 | GBM cancer cells | Astrocyte-like | EPR12686/abcam |
| SMA | Normal brain cells | Vasculature | 1A4/R&D |
| SNAI1 | GBM cancer cells | Epithelial to Mesenchymal Transition | AF3639/R&D |
| SOD2 | GBM cancer cells | Mesenchymal | EPR2560Y/abcam |
| SOX2 | GBM cancer cells | GBM stem-like cell | O30-678/Fluidigm |
| TGFbeta | GBM cancer cells | GBM stem-like cell | TW4-6H10/Fluidigm |
| TMEM119 | Immune cells | Microglia | HPA051870/sigma |
| TNC | GBM cancer cells | GBM stem-like cell | MAB2138/R&D |