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. 2023 Jun 15;13(6):2285–2306.

Figure 3.

Figure 3

The immune activation on reactive T-cell proliferation in MBT-2 tumor bearing mice by different treatments. The mixed lymphocyte reaction was performed using lymphocytes isolated from the spleens of MBT-2 or MB49 tumor bearing mice with different treatments as effector cells and co-cultured with target tumor cells or XUCs. A. Representative flow cytometry analysis of CFDA-SE dilution after 3 days of mixed cultures of CFDA-SE-labeled lymphocytes (effector cells) from MBT-2 tumor bearing mice when co-cultured with target MBT-2 cells or XUCs. B. Quantitation of proliferating lymphocytes responding to MBT-2 cells or XUCs. C. Representative flow cytometry analysis of CFDA-SE dilution after 3 days of mixed cultures of CFDA-SE-labeled lymphocytes (effector cells) from MB49 tumor bearing mice when co-cultured with target MB49 cells or XUCs. D. Quantitation of proliferating lymphocytes responding to MB49 cells or XUCs. Error bars represent SD. *P<0.05; **P<0.01; ***P<0.001.