Figure 8.

Xenogeneic urothelial cells (XUC) remain in the injected tumor site after intratumoral injection. LuminiCell labelled XUCs or non-labelled XUCs were intratumorally injected into MBT-2 tumors. A. Representative images of IVIS AIE dot labeled (red) XUCs at 0, 7, 14, and 21 days after the intratumoral injection of XUC cells with or without labelling. B. Representative section images of the injected site at 7, 14, and 21 days after injection of XUC cells. C. The presence of porcine mitochondrial DNAs in MBT-2 tumors was detected different time points after injection. DNA was isolated from tumors with vehicle control or intratumoral XUC treatment for real-time qPCR with primers specific for porcine mitochondrial cytochrome-b (Cytb) gene. Threshold cycles (Ct) were determined and plotted.